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KMID : 0882420100790050536
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Korean Journal of Medicine
2010 Volume.79 No. 5 p.536 ~ p.542
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Effects of glucosamine on Ca2+ signaling and K+ channel currents in T lymphocytes
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Suh Eun-Young
Pang Bo
Kim Sung-Joon
Kim Woo-Kyung
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Abstract
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Background/Aims: Glucosamine is widely taken as a functional food, and some studies reported its anti-inflammatory effects. K+ channels and intracellular signal play important roles in the activation of immune cells such as T lymphocytes. Therefore we aimed to examine the effects of glucosamine on the cell physiological parameters.
Methods: In Jurkat-T lymphocytes, intracellular [Ca2+] ([Ca2+]i) was measured using fura-2 fluorimetry, and voltage-gated K+ current (IKv) was measured using whole-cell clamp technique. Ca2+-activated K+ current (IKca) was measured in HEK293 cells over expressing SK4 using inside-out patch clamp technique.
Results: An acute application of glucosamine (0.5 mM) affected neither the increase in [Ca2+]i induced by CD3 stimulation (anti-CD3 Ab, 5 μg/mL) nor the IKv in Jurkat-T cells. A chronic stimulation of with anti-CD3 Ab (5 μg/mL, 24~36 hr) largely increased the amplitude of IKv. However, the combined treatment with glucosamine (0.1 mM) did not block the increase of IKv. The IKCa in SK4-overexpressing cells was slightly decreased by glucosamine (0.5 mM).
Conclusions: While glucosamine had a minor inhibitory effect on SK4 K+ channels, the anti-inflammatory effects of glucosamine could not be explained by the effects on the Ca2+ signaling in T lymphocytes.
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KEYWORD
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Glucosamine, Calcium, T cell, Ion channel
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